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BG11

medios de agua dulce


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BG11 Medium

(Allen 1968, Allen and Stainer 1968, Rippka et al. 1979)

#

Component

Stock solution

(g . L-1 dH2O)

Addition per 1 Litre culture medium

1

NaNO3

 

1.5 g

2

K2HPO4 . 3H2O

40.00

1 ml

3

MgSO4 . 7H2O

75.0

1 ml

4

CaCl2. 2H2O

36.0

1 ml

5

Citric Acid

6.0

1 ml

6

Ammonium Ferric Citrate

6.0

1 ml

7

MgNa2EDTA . H2O

1.0

1 ml

8

Na2CO3

20

1 ml

9

Trace Metals

 

1 ml

Preparation of Trace Metal Solution

#

Metal

Addition per 1 litre

bidistilled water

1

H3BO3

2.86 g

2

MnCl2 . 4H2O

1.81 g

3

ZnSO4 . 7H2O

0.22 g

4

Na2MoO4 . 2H2O

0.39 g

4

CuSO4 . 5H2O

0.08 g

6

Co(NO3)2 . 6H2O

0.05 g

Preparation of Culture Solution

Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. Adjust pH to 7.4 with NaOH and autoclave.