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Medios de cultivo Medios de cultivo

CM-0009
L1

55,00 €

    Culture Medium L1

    (Guillard & Hargraves 1993)

    #

    Component

    Stock solution

    (g L-1 dH2O)

    Addition per 1 Litre culture medium

    1

    NaNO3

    75 g

    1 ml

    2

    NaH2PO4 . 2H2O

    5 g

    1 ml

    3

    Trace metal

     

    1 ml

    4

    Vitamins

     

    0.5 ml

     

     Preparation of Trace Metal Solution

     

    #

    Component

    Stock solution

    (g . L-1)

    Addition per 1000 ml bidestilled water

    1

    Na2EDTA 2 H2O

     

    4.36 g

    2

    FeCl3 . 6 H2O

     

    3.15

    3

    K2CrO4

    1.94

    1 ml

    4

    CoCl2 . 6 H2O

    11.90

    1 ml

    5

    CuSO4 . 5 H2O

    2.50

    1 ml

    6

    MnCl2 . 4 H2O

    178.10

    1 ml

    7

    Na2MoO4 . 2 H2O

    19.90

    1 ml

    8

    NiSO4 . 6 H2O

    2.63

    1 ml

    9

    H2SeO3

    1.29

    1 ml

    10

    Na3VO4

    1.84

    1 ml

    11

    ZnSO4 . 7 H2O

    23.00

    1 ml

     

    Preparation of vitamins Solution

     

    #

    Vitamin

    Stock solution

    (g . L-1)

    Addition per 1 Litre stock vitamin solution

    1

    Biotin

    0.5

    1 ml

    2

    Thiamine-HCl 

     

    100 mg

    3

    Cyanocobalamin

    0.5

    1 ml

     

    Preparation of Culture Solution

     

    Add chemicals and stock solutions as indicated to 950 ml of filtered natural seawater and bring to final volume to 1 liter using filter natural seawater. Adjust pH to 8 with 1M NaOH.


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