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Medios de cultivo Medios de cultivo

CM-0004
BBM

55,00 €

    Bold´s Basal Medium (BBM)

    (Bold 1949, Bischoft & Bold 1963)

    #

    Component

    Stock solution

    (g . L-1 dH2O)

    Addition per 1 Litre culture medium

    1

    NaNO3

    25.00

    10 ml

    2

    CaCl2 . 2H2O

    2.50

    10 ml

    3

    MgSO4 . 7H2O

    7.50

    10 ml

    4

    K2HPO4

    7.50

    10 ml

    5

    KH2PO4

    17.50

    10 ml

    6

    NaCl

    2.50

    10 ml

    7

    Alkaline EDTA solution

    EDTA (Titriplex III)

    KOH

    50

    31 

    1 ml

    8

    Acidified Iron solution

    FeSO4 . 7 H2

    H2SO4

    4.98

    1 ml

    1ml

    9

    Boron solution

    H3BO3

    11.42

    1ml

    10

    Trace Metals solution

    ZnSO4 .7H2O

    MnCl2 .4H2O

    MoO3

    CuSO4 .5H2O

    Co(NO3)2 .6H2O

    8.82

    1.44

    0.71

    1.57

    0.49

    1ml

     

    Preparation of Culture Solution

     

    Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. The final pH should be 6.6.


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