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Medios de cultivo Medios de cultivo

CM-0002
ASP-12 + 2xNaCl

55,00 €

    ASP-12 Medium + 2 x NaCl

    Medium used to culture Dunaliella sps. Prepare ASP-12 culture medium as indicated below, (according to  J. McLachlan: Growth media – marine. In: Handbook of Phycological Methods, Culture Methods and Growth Measurements; ed.:  Janet R. Stein; pp. 25 – 52) however, add 58 g of NaCl.

    #

    Component

    Stock solution

    (g . L-1 dH2O)

    Addition per 1 Litre culture medium

    1

    HEPES

    238.10 g

    3 ml

    2

    NaCl

     

    58 g

    3

    KCl

    60.00 g

    11.6 ml

    4

    MgSO4 . 7 H2O

     

    7 g

    5

    MgCl2 . 6 H2O

     

    4 g

    6

    CaCl2 . 2 H2O

    370.00 g

    4 ml

    7

    NaNO3

    100.30 g

    1 ml

    8

    K3PO4 . 3 H2O

    12.50 g

    1 ml

    9

    Na2-Glycerophosphate

    6.85 g

    1 ml

    10

    Na2SiO3 . 9 H2O

    28.42 g

    5.3 ml

    11

    NTA (Titriplex I)*

    10.00 g

    10 ml

    12

    Vitamins

     

    1 ml

    13

    Trace Metals

     

    1 ml

     

    Preparation of Vitamins Solution

     

    #

    Component

    Stock solution concentration

    Addition per 1 Litre stock solution

    1

    Vitamin B12

    0.20 mg

    2

    Biotin

    1.00 mg

    3

    Thiamine-HCl

    100.00 mg

    4

    Niacinamide

    0.10 mg

    pH of this solution should be around pH 7.0

     

    Preparation of Trace Metal Solution

     

    #

    Component

    Stock solution

    (g . L-1)

    Addition per 1000 ml bidestilled water

    1

    Na2EDTA 2 H2O

     

    4.36 g

    2

    FeCl3 . 6 H2O

     

    3.15

    3

    K2CrO4

    1.94

    1 ml

    4

    CoCl2 . 6 H2O

    11.90

    1 ml

    5

    CuSO4 . 5 H2O

    2.50

    1 ml

    6

    MnCl2 . 4 H2O

    178.10

    1 ml

    7

    Na2MoO4 . 2 H2O

    19.90

    1 ml

    8

    NiSO4 . 6 H2O

    2.63

    1 ml

    9

    H2SeO3

    1.29

    1 ml

    10

    Na3VO4

    1.84

    1 ml

    11

    ZnSO4 . 7 H2O

    23.00

    1 ml

     

    Preparation of Culture Solution

     

    Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. Adjust pH to 7.9 with NaOH and autoclave.

    * Titriplex I  -  Nitrilotriacetic acid  -  C6H9NO6


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