Banco Espa├▒ol de Algas

CM-0017

Imprimir
No hay imagen establecida

SFM + Si

medios de agua dulce


Precio
Precio Base: 55,00 ÔéČ
Modificador de variaci├│n de precio:
Precio base con impuestos
Precio de venta con descuento:
Precio de venta55,00 ÔéČ
Descuento:
Cantidad de impuestos:
 

 

SFM + Si Medium

(Synthetic Freshwater Medium)

 

#

Component

Stock solution

Addition per 1 Litre stock solution

Addition per 1 Litre culture medium

1

Ca(NO3)2 . 4 H2O

100.00 g

0.5 ml

2

MgSO4 . 7 H2O

20.00 g

2.5 ml

3

K2HPO4 . 3 H2O

+ NaNO3

+ Na2CO3

5.00 g

50.00 g

32.00 g

0.6 ml

4

Vitamins

 

1 ml

5

HEPES

238.31 g

1 ml

6

Trace Metals

 

1 ml

7

Na2SiO3 9 H2O

28.42 g

5 ml

 

Preparation of Vitamins Solution

 

#

Vitamin

Stock solution concentration

Addition per 1 Litre stock solution

1

Vitamin B12

0.20 mg

2

Biotin

1.00 mg

3

Thiamine-HCl

100.00 mg

4

Niacinamide

0.10 mg

 

Preparation of Trace Metal Solution

 

#

Component

Stock solution

(g . L-1)

Addition per 1000 ml bidestilled water

1

Na2EDTA 2 H2O

 

4.36 g

2

FeCl3 . 6 H2O

 

3.15

3

K2CrO4

1.94

1 ml

4

CoCl2 . 6 H2O

11.90

1 ml

5

CuSO4 . 5 H2O

2.50

1 ml

6

MnCl2 . 4 H2O

178.10

1 ml

7

Na2MoO4 . 2 H2O

19.90

1 ml

8

NiSO4 . 6 H2O

2.63

1 ml

9

H2SeO3

1.29

1 ml

10

Na3VO4

1.84

1 ml

11

ZnSO4 . 7 H2O

23.00

1 ml

 

Preparation of Culture Solution

 

Add stock solutions 1-7 to 1000 ml of bidistilled water, adjust the pH to 7.0 and autoclave.

 

© 2017 Banco Espa├▒ol de Algas
cc