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Spirulina medium

medios salobres


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Precio Base: 55,00 ÔéČ
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Precio base con impuestos
Precio de venta con descuento:
Precio de venta55,00 ÔéČ
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Spirulina Medium, modified

(modified from Aiba and Ogawa 1977)

 

#

Component

Stock solution

Addition per 1 Litre culture medium

1

Solution I

NaHCO3

Na2CO3

K2HPO4

500 ml

13.61 g

4.03 g

0.50 g

2

Solution II

NaNO3

K2SO4

NaCl

MgSO4 ┬Ě 7H2O

CaCl2 ┬Ě 2H2O

FeSO4 ┬Ě 7H2O

Na2EDTA ┬Ě 2H2O

Trace metals solution

500 ml

2.5 g

1.0 g

1.0 g

0.2 g

0.04 g

0.01 g

0.08 g

1 ml

3

Vitamins solution

 

1ml

 

Preparation of Trace Metal Solution.

 

#

Component

Stock solution

(g 100 ml-1 dH2O)

Addition per 1 Litre final stock solution

1

Na2EDTA ┬Ě 2H2O

 

0.8 g

2

FeSO4 ┬Ě 7H2O

 

0.7 g

3

ZnSO4 ┬Ě 7H2O

0.1

1 ml

4

MnSO4 ┬Ě 7H2O

0.1

2 ml

5

H3BO3

0.2

5 ml

6

Co(NO3)2 ┬Ě 6H2O

0.02

5 ml

7

Na2MoO4 ┬Ě 2H2O

0.02

5 ml

8

CuSO4 ┬Ě 5H2O

0.0005

1ml

 

Dissolve the EDTA into 900 mLof dH2O and then dissolve each remaining component; bring the final volume to 1 liter.

 

Preparation of Vitamins Solution

 

#

Vitamin

Stock solution

Addition per 1 Litre

1

Vitamin B12

5 ┬Ě10-6 g

 

Preparation of Culture Solution.

 

Autoclave Solutions I and II separately and cool. Aseptically combine the two Solutions and add 1 mL of the vitamina B12 solution.

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