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Medios de cultivo Medios de cultivo

CM-0003
ASP-H

55,00 €

    ASP-H Medium, modified

    (G.I. McFadden & M. Melkonian 1986, Phycologia 25: 551-557)

    #

    Component

    Stock solution

    (g . L-1 dH2O)

    Addition per 1 Litre culture medium

    1

    NaCl

     

    18 g

    2

    MgSO4 . 7 H2O

     

    5 g

    3

    KCl

    60.00 g

    10 ml

    4

    HEPES

    238.31 g

    3 ml

    5

    CaCl2 . 2 H2O

    370.00 g

    1 ml

    6

    NaNO3

    + K2HPO4 . 3 H2O

    + Na2CO3

    50.00 g

    5.00 g

    30.00 g

    1 ml

    7

    NTA (Titriplex I)*

    NTA dissolves after addition of NaOH pellets and heating

    10.00 g

    1 ml

    8

    Fe-EDTA solution

     

    1ml

    9

    Trace Metals

     

    1 ml

    10

    H3BO3

    2.97 g

    10 ml

    11

    Vitamins solution

     

    1 ml

     

    Preparation of Fe-EDTA Solution

     

    #

    Component

    Stock solution concentration

    Addition per 1 Litre stock solution

    1

    EDTA (Titriplex II)*

    5.22 g

    2

    FeSO4 . 7 H2O

    4.98 g

    3

    1 N KOH

    54.00 ml

     

    EDTA (Titriplex II) and FeSO4 x 7 H2O is heated for 30 min (100oC); KOH is added to the cooled mixture

     

    Preparation of Trace Metal Solution

     

    #

    Component

    Stock solution concentration

    Addition per 1 Litre stock solution

    1

    ZnCl2

    0.33 g

    2

    MnCl2 . 4 H2O

    4.30 g

    3

    CoCl2. 6 H2O

    0.12 g

    4

    Na/EDTA (Titriplex III)*

    25.80g

    pH of Trace Metal Solution is adjusted to 7.5 with 10 N NaOH

    Preparation of Vitamins Solution

    #

    Component

    Stock solution concentration

    Addition per 1 Litre stock solution

    1

    Vitamin B12

    0.20 mg

    2

    Biotin

    1.00 mg

    3

    Thiamine-HCl

    100.00 mg

    4

    Niacinamide

    0.10 mg

    pH of this solution should be around pH 7.0

     

    Preparation of Culture Solution

     

    Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. Adjust pH to 7.7 with NaOH and autoclave.

    * Titriplex I  -    Nitrilotriacetic acid  -  C6H9NO6

    * Titriplex II  -   Ethylenediaminetetraacetic acid  -  C10H16N2O8

     *Titriplex III  -  Ethylenediaminetetraacetic acid  disodium salt dihydrate  - C10H14N2Na2O8 x 2 H2O


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