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Medios de cultivo Medios de cultivo

CM-0005
BG11

55,00 €

    BG11 Medium

    (Allen 1968, Allen and Stainer 1968, Rippka et al. 1979)

    #

    Component

    Stock solution

    (g . L-1 dH2O)

    Addition per 1 Litre culture medium

    1

    NaNO3

     

    1.5 g

    2

    K2HPO4 . 3H2O

    40.00

    1 ml

    3

    MgSO4 . 7H2O

    75.0

    1 ml

    4

    CaCl2. 2H2O

    36.0

    1 ml

    5

    Citric Acid

    6.0

    1 ml

    6

    Ammonium Ferric Citrate

    6.0

    1 ml

    7

    MgNa2EDTA . H2O

    1.0

    1 ml

    8

    Na2CO3

    20

    1 ml

    9

    Trace Metals

     

    1 ml

    Preparation of Trace Metal Solution

    #

    Metal

    Addition per 1 litre

    bidistilled water

    1

    H3BO3

    2.86 g

    2

    MnCl2 . 4H2O

    1.81 g

    3

    ZnSO4 . 7H2O

    0.22 g

    4

    Na2MoO4 . 2H2O

    0.39 g

    4

    CuSO4 . 5H2O

    0.08 g

    6

    Co(NO3)2 . 6H2O

    0.05 g

    Preparation of Culture Solution

    Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. Adjust pH to 7.4 with NaOH and autoclave.


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