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Medios de cultivo Medios de cultivo

CM-0008
F/2

55,00 €

    F2-media

    (Guillard & Ryther  1962, Guillard 1975)

     

    #

    Component

    Stock solution

    (g . L-1 dH2O)

    Addition per 1L

    bidistilled water

    1

    NaNO3

    75

    1 ml

    2

    NaH2PO4 . 2H2O

    5

    1 ml

    3

    Na2SiO3 . 9 H2O

    30

    1 ml

    4

    Trace elements solution

     

    1 ml

    5

    Vitamins solution

     

    0.5 ml

     

    Preparation of Trace Metal Solution

     

    #

    Component

    Addition per 1L

    bidistilled water

    1

    Na2EDTA

    0.436 g

    2

    FeCl3. 6H2O

    0.315 g

    3

    CuSO4. 5H2O

    0.010 g

    4

    ZnSO4 . 7H2O

    0.022 g

    5

    CoCl2 . 6H2O

    0.010 g

    6

    MnCl2 . 4H2O

    0.180 g

    7

    Na2MoO4 . 2H2O

    0.006 g

     

    Dissolve EDTA and other components into 1 liter with bidestilled water.

     

    Preparation of Vitamins Solution

     

    #

    Component

    1º Stock solution

    (g . L dH2O)

    Addition per 1L

    bidistilled water

    1

    Thiamine-HCl

     

    200 mg

    2

    Biotin

    1.00

    1 ml

    3

    Cyanocobalamin

    1.00

    1 ml

     

    Preparation of Culture Solution

     

    Add chemicals and stock solutions as indicated above to 1000 ml of filtered natural seawater water. Adjust pH to 8 with 1 M NaOH or HCl.


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BIOASIS

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