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Medios de cultivo Medios de cultivo

CM-0013
Spirulina medium

55,00 €

    Spirulina Medium, modified

    (modified from Aiba and Ogawa 1977)

     

    #

    Component

    Stock solution

    Addition per 1 Litre culture medium

    1

    Solution I

    NaHCO3

    Na2CO3

    K2HPO4

    500 ml

    13.61 g

    4.03 g

    0.50 g

    2

    Solution II

    NaNO3

    K2SO4

    NaCl

    MgSO4 · 7H2O

    CaCl2 · 2H2O

    FeSO4 · 7H2O

    Na2EDTA · 2H2O

    Trace metals solution

    500 ml

    2.5 g

    1.0 g

    1.0 g

    0.2 g

    0.04 g

    0.01 g

    0.08 g

    1 ml

    3

    Vitamins solution

     

    1ml

     

    Preparation of Trace Metal Solution.

     

    #

    Component

    Stock solution

    (g 100 ml-1 dH2O)

    Addition per 1 Litre final stock solution

    1

    Na2EDTA · 2H2O

     

    0.8 g

    2

    FeSO4 · 7H2O

     

    0.7 g

    3

    ZnSO4 · 7H2O

    0.1

    1 ml

    4

    MnSO4 · 7H2O

    0.1

    2 ml

    5

    H3BO3

    0.2

    5 ml

    6

    Co(NO3)2 · 6H2O

    0.02

    5 ml

    7

    Na2MoO4 · 2H2O

    0.02

    5 ml

    8

    CuSO4 · 5H2O

    0.0005

    1ml

     

    Dissolve the EDTA into 900 mLof dH2O and then dissolve each remaining component; bring the final volume to 1 liter.

     

    Preparation of Vitamins Solution

     

    #

    Vitamin

    Stock solution

    Addition per 1 Litre

    1

    Vitamin B12

    5 ·10-6 g

     

    Preparation of Culture Solution.

     

    Autoclave Solutions I and II separately and cool. Aseptically combine the two Solutions and add 1 mL of the vitamina B12 solution.


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SPEGC

Gobierno de Canarias

BIOASIS

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