CM-0002

Imprimir
No hay imagen establecida

ASP-12 + 2xNaCl

medios hipersalinos


Precio
Precio Base: 55,00 €
Modificador de variación de precio:
Precio base con impuestos
Precio de venta con descuento:
Precio de venta55,00 €
Descuento:
Cantidad de impuestos:
 

ASP-12 Medium + 2 x NaCl

Medium used to culture Dunaliella sps. Prepare ASP-12 culture medium as indicated below, (according to  J. McLachlan: Growth media – marine. In: Handbook of Phycological Methods, Culture Methods and Growth Measurements; ed.:  Janet R. Stein; pp. 25 – 52) however, add 58 g of NaCl.

#

Component

Stock solution

(g . L-1 dH2O)

Addition per 1 Litre culture medium

1

HEPES

238.10 g

3 ml

2

NaCl

 

58 g

3

KCl

60.00 g

11.6 ml

4

MgSO4 . 7 H2O

 

7 g

5

MgCl2 . 6 H2O

 

4 g

6

CaCl2 . 2 H2O

370.00 g

4 ml

7

NaNO3

100.30 g

1 ml

8

K3PO4 . 3 H2O

12.50 g

1 ml

9

Na2-Glycerophosphate

6.85 g

1 ml

10

Na2SiO3 . 9 H2O

28.42 g

5.3 ml

11

NTA (Titriplex I)*

10.00 g

10 ml

12

Vitamins

 

1 ml

13

Trace Metals

 

1 ml

 

Preparation of Vitamins Solution

 

#

Component

Stock solution concentration

Addition per 1 Litre stock solution

1

Vitamin B12

0.20 mg

2

Biotin

1.00 mg

3

Thiamine-HCl

100.00 mg

4

Niacinamide

0.10 mg

pH of this solution should be around pH 7.0

 

Preparation of Trace Metal Solution

 

#

Component

Stock solution

(g . L-1)

Addition per 1000 ml bidestilled water

1

Na2EDTA 2 H2O

 

4.36 g

2

FeCl3 . 6 H2O

 

3.15

3

K2CrO4

1.94

1 ml

4

CoCl2 . 6 H2O

11.90

1 ml

5

CuSO4 . 5 H2O

2.50

1 ml

6

MnCl2 . 4 H2O

178.10

1 ml

7

Na2MoO4 . 2 H2O

19.90

1 ml

8

NiSO4 . 6 H2O

2.63

1 ml

9

H2SeO3

1.29

1 ml

10

Na3VO4

1.84

1 ml

11

ZnSO4 . 7 H2O

23.00

1 ml

 

Preparation of Culture Solution

 

Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. Adjust pH to 7.9 with NaOH and autoclave.

* Titriplex I  -  Nitrilotriacetic acid  -  C6H9NO6