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Medios de cultivo Medios de cultivo

CM-0022
BBM + 3V

55,00 €

    Bold´s Basal Medium (BBM) + additional vitamins

    (modified Bold 1949, Bischoft & Bold 1963)

    #

    Component

    Final concentration

    Stock solution

    (g . L-1 dH2O)

    Addition per 1 Litre culture medium

    1

    NaNO3

    2.94 mM

    25.00

    10 ml

    2

    CaCl2·2H2O

    0.17 mM

    2.50

    10 ml

    3

    MgSO4·7H2O

    0.3 mM

    7.50

    10 ml

    4

    K2HPO4

    0.43 mM

    7.50

    10 ml

    5

    KH2PO4

    1.29 mM

    17.50

    10 ml

    6

    NaCl

    0.43 mM

    2.50

    10 ml

    7

    Alkaline EDTA solution

    EDTA (Titriplex III)

    KOH

    17.10 mM

    55.30 mM

    50 g

    31 g

    1 ml

    8

    Acidified Iron solution

    FeSO4 x 7 H2O

    H2SO4

    0.179 mM

    4.98

    1ml

    9

    Boron solution

    H3BO3

    18.50 mM

    11.42

    1ml

    10

    Vitamins

    See below

    -

    3 ml

    11

    Trace Metals solution

    ZnSO4 7H2O

    MnCl2 4H2O

    MoO3

    CuSO4 5H2O

    Co(NO3)2 6H2O

    8.82

    1.44

    0.71

    1.57

    0.49

    0.307 µM

    7.28 µM

    4.93 µM

    6.29 µM

    1.68 µM

    1ml

    Preparation of vitamins Solution

    #

    Vitamin

    Final concentration

    Stock solution concentration

    Addition per 1 Litre stock solution

    1

    Vitamin B12

    0.15 nM

    0.20 mg

    2

    Biotin

    4.10 nM

    1.00 mg

    3

    Thiamine-HCl

    0.30 µM

    100.00 mg

    4

    Niacinamide

    0.80 nM

    0.10 mg

    pH of this solution should be around pH 7

    Preparation of Culture Solution

    Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. The final pH should be 6.6.


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