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F/2

medios de agua de mar


Precio
Precio Base: 55,00 €
Modificador de variación de precio:
Precio base con impuestos
Precio de venta con descuento:
Precio de venta55,00 €
Descuento:
Cantidad de impuestos:
 

F2-media

(Guillard & Ryther  1962, Guillard 1975)

 

#

Component

Stock solution

(g . L-1 dH2O)

Addition per 1L

bidistilled water

1

NaNO3

75

1 ml

2

NaH2PO4 . 2H2O

5

1 ml

3

Na2SiO3 . 9 H2O

30

1 ml

4

Trace elements solution

 

1 ml

5

Vitamins solution

 

0.5 ml

 

Preparation of Trace Metal Solution

 

#

Component

Addition per 1L

bidistilled water

1

Na2EDTA

0.436 g

2

FeCl3. 6H2O

0.315 g

3

CuSO4. 5H2O

0.010 g

4

ZnSO4 . 7H2O

0.022 g

5

CoCl2 . 6H2O

0.010 g

6

MnCl2 . 4H2O

0.180 g

7

Na2MoO4 . 2H2O

0.006 g

 

Dissolve EDTA and other components into 1 liter with bidestilled water.

 

Preparation of Vitamins Solution

 

#

Component

1º Stock solution

(g . L dH2O)

Addition per 1L

bidistilled water

1

Thiamine-HCl

 

200 mg

2

Biotin

1.00

1 ml

3

Cyanocobalamin

1.00

1 ml

 

Preparation of Culture Solution

 

Add chemicals and stock solutions as indicated above to 1000 ml of filtered natural seawater water. Adjust pH to 8 with 1 M NaOH or HCl.