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Medios de cultivo Medios de cultivo

CM-0017
SFM + Si

55,00 €

     

    SFM + Si Medium

    (Synthetic Freshwater Medium)

     

    #

    Component

    Stock solution

    Addition per 1 Litre stock solution

    Addition per 1 Litre culture medium

    1

    Ca(NO3)2 . 4 H2O

    100.00 g

    0.5 ml

    2

    MgSO4 . 7 H2O

    20.00 g

    2.5 ml

    3

    K2HPO4 . 3 H2O

    + NaNO3

    + Na2CO3

    5.00 g

    50.00 g

    32.00 g

    0.6 ml

    4

    Vitamins

     

    1 ml

    5

    HEPES

    238.31 g

    1 ml

    6

    Trace Metals

     

    1 ml

    7

    Na2SiO3 9 H2O

    28.42 g

    5 ml

     

    Preparation of Vitamins Solution

     

    #

    Vitamin

    Stock solution concentration

    Addition per 1 Litre stock solution

    1

    Vitamin B12

    0.20 mg

    2

    Biotin

    1.00 mg

    3

    Thiamine-HCl

    100.00 mg

    4

    Niacinamide

    0.10 mg

     

    Preparation of Trace Metal Solution

     

    #

    Component

    Stock solution

    (g . L-1)

    Addition per 1000 ml bidestilled water

    1

    Na2EDTA 2 H2O

     

    4.36 g

    2

    FeCl3 . 6 H2O

     

    3.15

    3

    K2CrO4

    1.94

    1 ml

    4

    CoCl2 . 6 H2O

    11.90

    1 ml

    5

    CuSO4 . 5 H2O

    2.50

    1 ml

    6

    MnCl2 . 4 H2O

    178.10

    1 ml

    7

    Na2MoO4 . 2 H2O

    19.90

    1 ml

    8

    NiSO4 . 6 H2O

    2.63

    1 ml

    9

    H2SeO3

    1.29

    1 ml

    10

    Na3VO4

    1.84

    1 ml

    11

    ZnSO4 . 7 H2O

    23.00

    1 ml

     

    Preparation of Culture Solution

     

    Add stock solutions 1-7 to 1000 ml of bidistilled water, adjust the pH to 7.0 and autoclave.

     


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Entidades Colaboradoras

Unión Europea

SPEGC

Gobierno de Canarias

BIOASIS

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