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2021 comes to a close! During the Christmas break, the Online Store and request for services will remain closed. The last orders to be attended will be those that are made until 15th of December 2020 inclusive. Orders placed after the deadline will not ship until January 2022.

 

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Medios de cultivo Medios de cultivo

CM-0008
F/2

55,00 €

    F2-media

    (Guillard & Ryther  1962, Guillard 1975)

     

    #

    Component

    Stock solution

    (g . L-1 dH2O)

    Addition per 1L

    bidistilled water

    1

    NaNO3

    75

    1 ml

    2

    NaH2PO4 . 2H2O

    5

    1 ml

    3

    Na2SiO3 . 9 H2O

    30

    1 ml

    4

    Trace elements solution

     

    1 ml

    5

    Vitamins solution

     

    0.5 ml

     

    Preparation of Trace Metal Solution

     

    #

    Component

    Addition per 1L

    bidistilled water

    1

    Na2EDTA

    0.436 g

    2

    FeCl3. 6H2O

    0.315 g

    3

    CuSO4. 5H2O

    0.010 g

    4

    ZnSO4 . 7H2O

    0.022 g

    5

    CoCl2 . 6H2O

    0.010 g

    6

    MnCl2 . 4H2O

    0.180 g

    7

    Na2MoO4 . 2H2O

    0.006 g

     

    Dissolve EDTA and other components into 1 liter with bidestilled water.

     

    Preparation of Vitamins Solution

     

    #

    Component

    1º Stock solution

    (g . L dH2O)

    Addition per 1L

    bidistilled water

    1

    Thiamine-HCl

     

    200 mg

    2

    Biotin

    1.00

    1 ml

    3

    Cyanocobalamin

    1.00

    1 ml

     

    Preparation of Culture Solution

     

    Add chemicals and stock solutions as indicated above to 1000 ml of filtered natural seawater water. Adjust pH to 8 with 1 M NaOH or HCl.


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