Culture Medium L1
(Guillard & Hargraves 1993)
# |
Component |
Stock solution (g L-1 dH2O) |
Addition per 1 Litre culture medium |
1 |
NaNO3 |
75 g |
1 ml |
2 |
NaH2PO4 . 2H2O |
5 g |
1 ml |
3 |
Trace metal |
1 ml |
|
4 |
Vitamins |
0.5 ml |
Preparation of Trace Metal Solution
# |
Component |
Stock solution (g . L-1) |
Addition per 1000 ml bidestilled water |
1 |
Na2EDTA . 2 H2O |
4.36 g |
|
2 |
FeCl3 . 6 H2O |
3.15 |
|
3 |
K2CrO4 |
1.94 |
1 ml |
4 |
CoCl2 . 6 H2O |
11.90 |
1 ml |
5 |
CuSO4 . 5 H2O |
2.50 |
1 ml |
6 |
MnCl2 . 4 H2O |
178.10 |
1 ml |
7 |
Na2MoO4 . 2 H2O |
19.90 |
1 ml |
8 |
NiSO4 . 6 H2O |
2.63 |
1 ml |
9 |
H2SeO3 |
1.29 |
1 ml |
10 |
Na3VO4 |
1.84 |
1 ml |
11 |
ZnSO4 . 7 H2O |
23.00 |
1 ml |
Preparation of vitamins Solution
# |
Vitamin |
Stock solution (g . L-1) |
Addition per 1 Litre stock vitamin solution |
1 |
Biotin |
0.5 |
1 ml |
2 |
Thiamine-HCl |
100 mg |
|
3 |
Cyanocobalamin |
0.5 |
1 ml |
Preparation of Culture Solution
Add chemicals and stock solutions as indicated to 950 ml of filtered natural seawater and bring to final volume to 1 liter using filter natural seawater. Adjust pH to 8 with 1M NaOH.
Strains
60.00€
Culture media
55.00€
Strains
150.00€
Culture media
55.00€
RedSys Bankia Gateway
72 hours maximum
Public enterprises.
+34 928 133 290