Bold´s Basal Medium (BBM)
(Bold 1949, Bischoft & Bold 1963)
# |
Component |
Stock solution (g . L-1 dH2O) |
Addition per 1 Litre culture medium |
1 |
NaNO3 |
25.00 |
10 ml |
2 |
CaCl2 . 2H2O |
2.50 |
10 ml |
3 |
MgSO4 . 7H2O |
7.50 |
10 ml |
4 |
K2HPO4 |
7.50 |
10 ml |
5 |
KH2PO4 |
17.50 |
10 ml |
6 |
NaCl |
2.50 |
10 ml |
7 |
Alkaline EDTA solution EDTA (Titriplex III) KOH |
50 31 |
1 ml |
8 |
Acidified Iron solution FeSO4 . 7 H2O H2SO4 |
4.98 1 ml |
1ml |
9 |
Boron solution H3BO3 |
11.42 |
1ml |
10 |
Trace Metals solution ZnSO4 .7H2O MnCl2 .4H2O MoO3 CuSO4 .5H2O Co(NO3)2 .6H2O |
8.82 1.44 0.71 1.57 0.49 |
1ml |
Preparation of Culture Solution
Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. The final pH should be 6.6.
Strains
60.00€
Culture media
55.00€
Strains
150.00€
Culture media
55.00€
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