CM-0013
Spirulina medium

#

Component

Stock solution

Addition per 1 Litre culture medium

1

Solution I

NaHCO₃

Na₂CO₃

K₂HPO₄

500 ml

13.61 g

4.03 g

0.50 g

2

Solution II

NaNO₃

K₂SO₄

NaCl

MgSO₄ · 7H₂O

CaCl₂ · 2H₂O

FeSO₄ · 7H₂O

Na₂EDTA · 2H₂O

Trace metals solution

500 ml

2.5 g

1.0 g

1.0 g

0.2 g

0.04 g

0.01 g

0.08 g

1 ml

3

Vitamins solution

1ml

#

Component

Stock solution

(g 100 ml^-1 dH₂O)

Addition per 1 Litre final stock solution

1

Na₂EDTA · 2H₂O

0.8 g

2

FeSO₄ · 7H₂O

0.7 g

3

ZnSO₄ · 7H₂O

0.1

1 ml

4

MnSO₄ · 7H₂O

0.1

2 ml

5

H₃BO₃

0.2

5 ml

6

Co(NO₃)₂ · 6H₂O

0.02

5 ml

7

Na₂MoO₄ · 2H₂O

0.02

5 ml

8

CuSO₄ · 5H₂O

0.0005

1ml

#

Vitamin

Stock solution

Addition per 1 Litre

1

Vitamin B₁₂

5 ·10^-6 g