CM-0001
ASP-12

#

Component

Stock solution

(g ^. L^-1 dH₂O)

Addition per 1 Litre culture medium

1

HEPES

238.10 g

3 ml

2

NaCl

28 g

3

KCl

60.00 g

11.6 ml

4

MgSO₄ ^. 7 H₂O

7 g

5

MgCl₂ ^. 6H₂O

4 g

6

CaCl₂ ^. 2H₂O

370.00 g

4 ml

7

NaNO₃

100.30 g

1 ml

8

K₃PO₄ ^. 3H₂O

12.50 g

1 ml

9

Na₂-Glycerophosphate

6.85 g

1 ml

10

Na₂SiO₃ ^. 9H₂O

28.42 g

5.3 ml

11

NTA (Titriplex I)*

10.00 g

10 ml

12

Vitamins

1 ml

13

Trace Metals

1 ml

#

Component

Stock solution concentration

Addition per 1 Litre stock solution

1

Vitamin B₁₂

0.20 mg

2

Biotin

1.00 mg

3

Thiamine-HCl

100.00 mg

4

Niacinamide

0.10 mg

#

Component

Stock solution

(g . L^-1)

Addition per 1000 ml bidistilled water

1

Na₂EDTA ^.2H₂O

4.36 g

2

FeCl₃ ^. 6H₂O

3.15

3

K₂CrO₄

1.94

1 ml

4

CoCl₂ ^. 6H₂O

11.90

1 ml

5

CuSO₄ ^. 5H₂O

2.50

1 ml

6

MnCl₂ ^. 4H₂O

178.10

1 ml

7

Na₂MoO₄ ^. 2H₂O

19.90

1 ml

8

NiSO₄ ^. 6H₂O

2.63

1 ml

9

H₂SeO₃

1.29

1 ml

10

Na₃VO₄

1.84

1 ml

11

ZnSO₄ ^. 7H₂O

23.00

1 ml