F2-media
(Guillard & Ryther 1962, Guillard 1975)
# |
Component |
Stock solution (g . L-1 dH2O) |
Addition per 1L bidistilled water |
1 |
NaNO3 |
75 |
1 ml |
2 |
NaH2PO4 . 2H2O |
5 |
1 ml |
3 |
Na2SiO3 . 9 H2O |
30 |
1 ml |
4 |
Trace elements solution |
1 ml |
|
5 |
Vitamins solution |
0.5 ml |
Preparation of Trace Metal Solution
# |
Component |
Addition per 1L bidistilled water |
1 |
Na2EDTA |
0.436 g |
2 |
FeCl3. 6H2O |
0.315 g |
3 |
CuSO4. 5H2O |
0.010 g |
4 |
ZnSO4 . 7H2O |
0.022 g |
5 |
CoCl2 . 6H2O |
0.010 g |
6 |
MnCl2 . 4H2O |
0.180 g |
7 |
Na2MoO4 . 2H2O |
0.006 g |
Dissolve EDTA and other components into 1 liter with bidestilled water.
Preparation of Vitamins Solution
# |
Component |
1º Stock solution (g . L dH2O) |
Addition per 1L bidistilled water |
1 |
Thiamine-HCl |
200 mg |
|
2 |
Biotin |
1.00 |
1 ml |
3 |
Cyanocobalamin |
1.00 |
1 ml |
Preparation of Culture Solution
Add chemicals and stock solutions as indicated above to 1000 ml of filtered natural seawater water. Adjust pH to 8 with 1 M NaOH or HCl.
Cepas
60.00€
Medios
55.00€
Cepas
150.00€
Medios
55.00€
Pasarela RedSys Bankia
Empresas públicas.
+34 928 133 290