CM-0022
BBM + 3V

#

Component

Final concentration

Stock solution

(g . L^-1 dH₂O)

Addition per 1 Litre culture medium

1

NaNO₃

2.94 mM

25.00

10 ml

2

CaCl₂·2H₂O

0.17 mM

2.50

10 ml

3

MgSO₄·7H₂O

0.3 mM

7.50

10 ml

4

K₂HPO₄

0.43 mM

7.50

10 ml

5

KH₂PO₄

1.29 mM

17.50

10 ml

6

NaCl

0.43 mM

2.50

10 ml

7

Alkaline EDTA solution

EDTA (Titriplex III)

KOH

17.10 mM

55.30 mM

50 g

31 g

1 ml

8

Acidified Iron solution

FeSO₄ x 7 H₂O

H₂SO₄

0.179 mM

4.98

1ml

9

Boron solution

H₃BO₃

18.50 mM

11.42

1ml

10

Vitamins

See below

-

3 ml

11

Trace Metals solution

ZnSO₄ 7H₂O

MnCl₂ 4H₂O

MoO₃

CuSO₄ 5H₂O

Co(NO₃)₂ 6H₂O

8.82

1.44

0.71

1.57

0.49

0.307 µM

7.28 µM

4.93 µM

6.29 µM

1.68 µM

1ml

#

Vitamin

Final concentration

Stock solution concentration

Addition per 1 Litre stock solution

1

Vitamin B₁₂

0.15 nM

0.20 mg

2

Biotin

4.10 nM

1.00 mg

3

Thiamine-HCl

0.30 µM

100.00 mg

4

Niacinamide

0.80 nM

0.10 mg