BG11 Medium
(Allen 1968, Allen and Stainer 1968, Rippka et al. 1979)
# |
Component |
Stock solution (g . L-1 dH2O) |
Addition per 1 Litre culture medium |
1 |
NaNO3 |
1.5 g |
|
2 |
K2HPO4 . 3H2O |
40.00 |
1 ml |
3 |
MgSO4 . 7H2O |
75.0 |
1 ml |
4 |
CaCl2. 2H2O |
36.0 |
1 ml |
5 |
Citric Acid |
6.0 |
1 ml |
6 |
Ammonium Ferric Citrate |
6.0 |
1 ml |
7 |
MgNa2EDTA . H2O |
1.0 |
1 ml |
8 |
Na2CO3 |
20 |
1 ml |
9 |
Trace Metals |
1 ml |
Preparation of Trace Metal Solution
# |
Metal |
Addition per 1 litre bidistilled water |
1 |
H3BO3 |
2.86 g |
2 |
MnCl2 . 4H2O |
1.81 g |
3 |
ZnSO4 . 7H2O |
0.22 g |
4 |
Na2MoO4 . 2H2O |
0.39 g |
4 |
CuSO4 . 5H2O |
0.08 g |
6 |
Co(NO3)2 . 6H2O |
0.05 g |
Preparation of Culture Solution
Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. Adjust pH to 7.4 with NaOH and autoclave.
Cepas
60.00€
Medios
55.00€
Cepas
150.00€
Medios
55.00€
Pasarela RedSys Bankia
Empresas públicas.
+34 928 133 290