Waris-H + additional vitamins
(McFadden G.I & Melkonian M., 1986)
# |
Component |
Stock solution Addition per 1 Litre stock solution |
Addition per 1 Litre culture medium |
1 |
KNO3 |
100.00 g |
1 ml |
2 |
MgSO4 . 7 H2O |
20.00 g |
1 ml |
3 |
(NH4)2HPO4 |
20.00 g |
1 ml |
4 |
Ca(NO3)2 . 4 H2O |
100.00 g |
1 ml |
5 |
HEPES |
238.31 g |
1 ml |
6 |
P-II Metals |
1 ml |
|
7 |
Fe-EDTA |
1ml |
|
8 |
Vitamins |
3 ml |
|
9 |
Soil extract |
10 ml |
Preparation of PII Metals Solution
# |
Component |
Stock solution Addition per 1 Litre stock solution |
1 |
EDTA (Titriplex III)* |
3.00 g |
2 |
H3BO3 |
1.14 g |
3 |
MnCl2 . 4 H2O |
0.144 g |
4 |
ZnSO4 . 7 H2O |
0.021 g |
5 |
CoCl2 . 6 H2O |
0.004 g |
Preparation of Fe-EDTA Solution
# |
Component |
Stock solution concentration Addition per 1 Litre stock solution |
1 |
EDTA (Titriplex II)* |
5.22 g |
2 |
FeSO4 . 7 H2O |
4.98 g |
3 |
1 N KOH |
54.00 ml |
EDTA (Titriplex II) and FeSO4 x 7 H2O is heated for 30 min (100oC); KOH is added to the cooled mixture
Preparation of Vitamins Solution
# |
Vitamin |
Stock solution concentration Addition per 1 Litre stock solution |
1 |
Vitamin B12 |
0.20 mg |
2 |
Biotin |
1.00 mg |
3 |
Thiamine-HCl |
100.00 mg |
4 |
Niacinamide |
0.10 mg |
Preparation of Soil Extract
10g of garden-soil is mixed with 120 ml bidistilled water and boiled for 10 minutes. Afterwards it is centrifuged for 10 minutes (low speed), and the supernatant is filtered through a series of membrane filters from 1.2 µm – 0.1 µm pore size. The remaining filtrate is adjusted to 100 ml with bidistilled water. Aliquots of 10 ml are stored frozen. The soil should not be recently fertilized and should not contain too much humus.
Preparation of Culture Solution
Add 1 ml of stock solutions 1-8 to 1000 ml of bidistilled water. Add 10 ml of thawed soil extract (= stock solution 9), adjust the pH to 7.0 and autoclave.
* Titriplex II - Ethylenediaminetetraacetic acid - C10H16N2O8
* Titriplex III - Ethylenediaminetetraacetic acid disodium salt dihydrate - C10H14N2Na2O8 x 2 H2O
Cepas
60.00€
Medios
55.00€
Cepas
150.00€
Medios
55.00€
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