CM-0003
ASP-H

#

Component

Stock solution

(g ^. L^-1 dH₂O)

Addition per 1 Litre culture medium

1

NaCl

18 g

2

MgSO₄ ^. 7 H₂O

5 g

3

KCl

60.00 g

10 ml

4

HEPES

238.31 g

3 ml

5

CaCl₂ ^. 2 H₂O

370.00 g

1 ml

6

NaNO₃

+ K₂HPO₄ ^. 3 H₂O

+ Na₂CO₃

50.00 g

5.00 g

30.00 g

1 ml

7

NTA (Titriplex I)*

NTA dissolves after addition of NaOH pellets and heating

10.00 g

1 ml

8

Fe-EDTA solution

1ml

9

Trace Metals

1 ml

10

H₃BO₃

2.97 g

10 ml

11

Vitamins solution

1 ml

#

Component

Stock solution concentration

Addition per 1 Litre stock solution

1

EDTA (Titriplex II)*

5.22 g

2

FeSO₄ ^. 7 H₂O

4.98 g

3

1 N KOH

54.00 ml

#

Component

Stock solution concentration

Addition per 1 Litre stock solution

1

ZnCl₂

0.33 g

2

MnCl₂ ^. 4 H₂O

4.30 g

3

CoCl₂^. 6 H₂O

0.12 g

4

Na/EDTA (Titriplex III)*

25.80g

#

Component

Stock solution concentration

Addition per 1 Litre stock solution

1

Vitamin B₁₂

0.20 mg

2

Biotin

1.00 mg

3

Thiamine-HCl

100.00 mg

4

Niacinamide

0.10 mg