Spirulina Medium, modified
(modified from Aiba and Ogawa 1977)
# |
Component |
Stock solution |
Addition per 1 Litre culture medium |
1 |
Solution I NaHCO3 Na2CO3 K2HPO4 |
500 ml |
13.61 g 4.03 g 0.50 g |
2 |
Solution II NaNO3 K2SO4 NaCl MgSO4 · 7H2O CaCl2 · 2H2O FeSO4 · 7H2O Na2EDTA · 2H2O Trace metals solution |
500 ml |
2.5 g 1.0 g 1.0 g 0.2 g 0.04 g 0.01 g 0.08 g 1 ml |
3 |
Vitamins solution |
1ml |
Preparation of Trace Metal Solution.
# |
Component |
Stock solution (g 100 ml-1 dH2O) |
Addition per 1 Litre final stock solution |
1 |
Na2EDTA · 2H2O |
0.8 g |
|
2 |
FeSO4 · 7H2O |
0.7 g |
|
3 |
ZnSO4 · 7H2O |
0.1 |
1 ml |
4 |
MnSO4 · 7H2O |
0.1 |
2 ml |
5 |
H3BO3 |
0.2 |
5 ml |
6 |
Co(NO3)2 · 6H2O |
0.02 |
5 ml |
7 |
Na2MoO4 · 2H2O |
0.02 |
5 ml |
8 |
CuSO4 · 5H2O |
0.0005 |
1ml |
Dissolve the EDTA into 900 mLof dH2O and then dissolve each remaining component; bring the final volume to 1 liter.
Preparation of Vitamins Solution
# |
Vitamin |
Stock solution Addition per 1 Litre |
1 |
Vitamin B12 |
5 ·10-6 g |
Preparation of Culture Solution.
Autoclave Solutions I and II separately and cool. Aseptically combine the two Solutions and add 1 mL of the vitamina B12 solution.
Cepas
60.00€
Medios
55.00€
Cepas
150.00€
Medios
55.00€
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