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BEA will close its online store and services from December 15th to January 15th for the winter vacations. Only orders and services requested before Novembre 30th will be processed. Requests received after November 30th will be handled starting January 15.

 

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Medios de cultivo Medios de cultivo

CM-0001
ASP-12

55,00 €

     

    ASP-12 Medium, modified

     (according to  J. McLachlan: Growth media – marine. In: Handbook of Phycological Methods, Culture Methods and Growth Measurements; ed.:  Janet R. Stein; pp. 25 – 52)

     

    #

    Component

    Stock solution

    (g . L-1 dH2O)

    Addition per 1 Litre culture medium

    1

    HEPES

    238.10 g

    3 ml

    2

    NaCl

     

    28 g

    3

    KCl

    60.00 g

    11.6 ml

    4

    MgSO4 . 7 H2O

     

    7 g

    5

    MgCl2 . 6H2O

     

    4 g

    6

    CaCl2 . 2H2O

    370.00 g

    4 ml

    7

    NaNO3

    100.30 g

    1 ml

    8

    K3PO4 . 3H2O

    12.50 g

    1 ml

    9

    Na2-Glycerophosphate

    6.85 g

    1 ml

    10

    Na2SiO3 . 9H2O

    28.42 g

    5.3 ml

    11

    NTA (Titriplex I)*

    10.00 g

    10 ml

    12

    Vitamins

     

    1 ml

    13

    Trace Metals

     

    1 ml

     

    Preparation of Vitamins Solution

     

    #

    Component

    Stock solution concentration

    Addition per 1 Litre stock solution

    1

    Vitamin B12

    0.20 mg

    2

    Biotin

    1.00 mg

    3

    Thiamine-HCl

    100.00 mg

    4

    Niacinamide

    0.10 mg

     

    Preparation of Trace Metal Solution

     

    #

    Component

    Stock solution

    (g . L-1)

    Addition per 1000 ml bidistilled water

    1

    Na2EDTA .2H2O

     

    4.36 g

    2

    FeCl3 6H2O

     

    3.15

    3

    K2CrO4

    1.94

    1 ml

    4

    CoCl2 . 6H2O

    11.90

    1 ml

    5

    CuSO4 . 5H2O

    2.50

    1 ml

    6

    MnCl2 . 4H2O

    178.10

    1 ml

    7

    Na2MoO4 . 2H2O

    19.90

    1 ml

    8

    NiSO4 . 6H2O

    2.63

    1 ml

    9

    H2SeO3

    1.29

    1 ml

    10

    Na3VO4

    1.84

    1 ml

    11

    ZnSO4 . 7H2O

    23.00

    1 ml

     

    Preparation of Culture Solution

     

    Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. Adjust pH to 7.9 with NaOH and autoclave.

    * Titriplex I  -  Nitrilotriacetic acid  -  C6H9NO6

     

     


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